MLAs have been told that mandatory Covid certification is for the "overall protection of the people of Northern Ireland" as they backed the regulations.
Health minister Robin Swann also issued a plea for unity over the messaging coming out of Stormont in the fight against Covid-19.
Following a Stormont debate which divided MLAs, the regulations were passed by 59 votes to 24. The scheme, which requires people to prove Covid status to gain entry to a range of hospitality venues and large-attendance events, was introduced late last month with a two-week grace period to allow businesses to adjust to the new requirements.
Patrons wishing to access nightclubs, pubs, restaurants and other licensed premises will need to provide proof of vaccination